Influence of Vitamin D Binding Protein on Accuracy of 25-Hydroxyvitamin D Measurement Using the ADVIA Centaur Vitamin D Total Assay

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Influence of Vitamin D Binding Protein on Accuracy of 25-Hydroxyvitamin D Measurement Using the ADVIA Centaur Vitamin D Total Assay

Vitamin D status in different populations relies on accurate measurement of total serum 25-hydroxyvitamin D [25(OH)D] concentrations [i.e., 25(OH)D3 and 25(OH)D2]. This study evaluated agreement between the ADVIA Centaur Vitamin D Total assay for 25(OH)D testing (traceable to the NIST-Ghent reference method procedure) and a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for va...

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Accuracy of 6 routine 25-hydroxyvitamin D assays: influence of vitamin D binding protein concentration.

BACKGROUND Recent recognition of its broad pathophysiological importance has triggered an increased interest in 25-hydroxyvitamin D [25(OH)D]. By consequence, throughput in 25(OH)D testing has become an issue for clinical laboratories, and several automated assays for measurement of 25(OH)D are now available. The aim of this study was to test the accuracy and robustness of these assays by compa...

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Outi Itkonen 2,3* Influence of Vitamin D 2 Percentage on Accuracy of 4 Commercial Total 25- Hydroxyvitamin D Assays

25-Hydroxyvitamin D (25-OHD) is the most appropriate marker for monitoring vitamin D storage status. The 2 major forms of 25OH-D [25-OH-D2 (D2) and 25OH-D3 (D3)], differ slightly in molecular structures but have essentially the same physiological activity. Therefore, both D2 and D3 should be measured to assess vitamin D status. At our laboratory, 42% of patient samples submitted for vitamin D t...

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Vitamin D Binding Protein Impact on 25-Hydroxyvitamin D Levels under Different Physiologic and Pathologic Conditions

There is a high prevalence of vitamin D deficiency worldwide, but how to define vitamin D deficiency is controversial. Currently, the plasma concentration of total 25-hydroxyvitamin D [25(OH)D] is considered an indicator of vitamin D status. The free hormone hypothesis states that protein-bound hormones are inactive while unbound hormones are free to exert biological activity. The majority of c...

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ژورنال

عنوان ژورنال: International Journal of Endocrinology

سال: 2014

ISSN: 1687-8337,1687-8345

DOI: 10.1155/2014/691679